乳腺癌组织CD44^＋CD24^-/low细胞含量检测及其临床意义

Detection of CD44^＋CD24^-/low in breast cancer stem cells and clinical significance

目的 探讨乳腺癌组织中CD44^＋CD24^-/low干细胞比例与各项临床指标的相关性.方法 新鲜乳腺癌组织标本68例.机械-酶法制成细胞悬液,流式细胞术检测CD44＋CD24-/loe细胞的含量,探讨其含量与肿瘤分期、免疫组织化学、远处转移等的相关性.结果 （1）68例乳腺癌组织中的CD44^＋CD24^-/low腺癌干细胞比例为0.1%～15.2%.（2）除ER组和远处转移组CD44^＋CD24^-/low细胞的含量差异有统计学意义（P〈0.05）外,年龄、肿瘤分期、腋窝淋巴结转移等组别,CD44^＋CD24^-/low细胞的含量差异均无统计学意义（P〉0.05）.结论 CD44^＋CD24^-/low乳腺癌干细胞比例与ER阴性表达、癌肿侵袭性和预后等临床指标明显相关.

Objective To investigate the relationship between CD44＋ CD24-/low breast cancer stem cells and clinical significance. Methods The fresh samples of 68 cases of breast cancer were collected , and single cell suspension was made by machine-enzymic method. The proportion of CD44^＋CD24^-/low cells was detected by flow cytometry, and its relationship with tumor stages, immunohistochemistry results and distant metastasis was analyzed. Results Among the 68 cases of breast cancer tissue, the proportion of CD44＋ CD24-/low cells was between 0.1% to 15. 2% , the difference of CD44＋ CD24-low cells content have statistical significance in ER status and metastasis team （P〈0. 05）, all of other groups （age, tumor stages,axillary nodes status and so on）are no statistical significance （P〉0. 05）. Conclusion There are relationships between CD44＋ CD24-/low breast cancer stem cells content and ER status,tumor invasion and prognosis.