自体负载肿瘤抗原树突状细胞治疗大鼠甲状腺癌

Immunotherapeutic effects of auto dendritic cells loaded with tumor lysates on rat transitional thyroid carcinoma

目的 观察自体负载肿瘤抗原树突状细胞（DC）对大鼠甲状腺癌的治疗作用.方法 建立Wistar荷瘤大鼠动物模型,随机分为治疗组和对照组,治疗组静脉输入负载肿瘤抗原DC、对照组输入生理盐水,检测分析2组血清中自细胞介素（IL）-12、IL-8表达水平,以及瘤重、瘤体体积及大鼠存活.结果 治疗组与对照组瘤重、瘤体体积、存活期、IL-12及IL-8表达水平分别为：（3.56±0.79）g比（4.97±0.56）g、（3.98±0.84）cm^3比（5.06±0.58）cm^3、（36.0±3.0）d比（28.0±3.2）d、（368.9±21.7）ng/L比（227.7±13.4）ng/L和（218.9±19.3）ng/L比（371.2±24.1）ng/L（P值分别〈0.01、〈0.01、〈0.05、〈0.01、〈0.01）.结论 自体负载肿瘤抗原DC对甲状腺癌具有较好的抑瘤效应,可延长荷瘤鼠的生存期.

Objective To investigate the immunotherapeutic effects of auto dendritic cells （DC） loaded with tumor lysates on rat transitional thyroid carcinoma. Methods After the thyroid tumor models in Wistar rats were successfully established, all rats were divided randomly into the treatment group and the control group. DC loaded with tumor lysates were injected into the tail vein of the rats in the treatment group, and the rats in control group, normal saline was injected into the tail vein. The levels of interleukin （IL）-12 and IL-8 in serum, the tumor weight and volume, and the life span of the rats were measured. Results The average tumor weight and volume, the life span, the levels of IL-12 and IL-8 in the treatment group and the control group were respectively （3. 56±0. 79） g vs （4. 97±0. 56） g, （3. 98±0. 84） cm^3 vs （5.06±0.58） cm^3, （36.0±3.0） days vs （28.0±3.2） days, （368.9±21.7） ng/L vs （227.7±13.4） ng/L, （218.9±19.3） ng/L vs （371.2±24. 1） ng/L. P values 〈0.01, 〈0.01, 〈0.05,〈0.01, 〈0.01, respectively. Conclusion Auto DC loaded with tumor lysates can inhibit tumor growth and prolong the survival time of Wistar rats with thyroid carcinoma.