肝细胞癌中HBx的表达及其与肿瘤病理分级的关系

The expression of HBx in hepatocellular carcinoma and its relationship with tumor pathological grading

目的 检测乙型肝炎病毒x蛋白（HBx）在肝细胞癌中的表达并探讨其与肝癌病理分级的关系.方法 用免疫组织化学法检测41例肝癌患者肿瘤组织中HBx蛋白的表达,与临床资料进行相关分析,并应用逆转录-聚合酶链反应（RT-PCR）、PCR及Western blot法检测肝癌患者肿瘤组织及血清中HBx的表达.结果 肝癌组织中HBx的总体阳性检出率为27/41（65.85%）,HBx＋与HBx-者肿瘤组织低分化比例分别为11/27（40.74%）及2/14（14.29%）,差异有统计学意义（P〈0.01）,且肿瘤组织低分化比例随HBx阳性表达程度的增加而增加,HBx强阳性表达者肿瘤组织低分化比例高达4/6（66.67%）;RT-PCR对肿瘤组织HBx mRNA的检出率为10/16（62.50%）,PCR对血清中HBx拷贝的检出率为1/7（14.29%）,Western blot对血清中HBx蛋白的检出率则为6/7（85.71%）.结论 肝癌肿瘤组织中HBx的表达与肿瘤的病理分级呈负相关,肿瘤组织低分化比例随HBx阳性表达程度的增加而增加.

Objective We aim to detect the expression of Hepatitis B virus X protein （HBx） in hepatocellular carcinoma （HCC） and investigate its relationship with tumor pathological grading. Methods Immunohistochemical method was utilized to detect the expression of HBx protein in tumor tissues of fortyone HCC patients,and the result was analyzed with the clinical data. Reverse transcription-polymerase chain reaction （RT-PCR） ,PCR,and Western blotting were also applied to detect the expression of HBx in tumor tissues and serum of HCC patients. Results The total ratio of HBx positive expression was 27/41 （65. 85%）. The poorly differentiated ratios of HBx positive and negative tumor tissues were 11/27 （40. 74%） and 2/14 （14. 29%） respectively, the difference was statistically significant （P〈0. 01）. And the poorly differentiated ratio of tumor tissues increased with the expression level of HBx, the positive ratio reached 4/6（66. 67%） when HBx was strongly positively expressed. RT-PCR could detect the mRNA of HBx in tumor tissues and the positive ratio was 10/16（62. 50%）. Although PCR could detect the HBx copy in the serum of HCC patients,the positive ratio was only 1/7（14. 29%）. And Western blotting could efficientlydetect HBx protein in HCC patients＇ serum,with the highest positive ratio 6/7（85.71%）. Conclusion HBx can be detected in both tumor tissues and serum of HCC patients. The expression of HBx is negatively correlated with pathological grading of HCC tumor tissues and the poorly differentiated ratio of tumor tissues increases with the expression level of HBx.