改进型左侧精索静脉曲张对大鼠睾丸酶及增殖细胞核抗原的影响

Effects of improved varicocele on testis enzymes and proliferating cell nuclear antigen in rats

目的 观察改进型左侧精索静脉曲张（ELV）对大鼠睾丸酶及增殖细胞核抗原（PCNA）的影响.方法 利用改进的方法建立青春期SD大鼠左精索静脉曲张的模型21只;假手术组SD大鼠15只作对照组.术后3个月,取部分睾丸组织匀浆提取上清液,比色法定量检测乳酸脱氢酶（LDH）、琥珀酸脱氢酶（SDH）、葡萄糖-6-磷酸脱氢酶（G6PDH）活性,利用酶联免疫吸附试验（ELISA）测定PCNA的浓度.结果 实验组左睾丸内的LDH、G6PDH活性分别为（8.17±3.47）、（34.00±16.29）U/mg,与对照组同侧（11.98±2.26）、（54.88±20.87）U/mg比较下降,与实验组右睾丸（12.69±3.97）、（78.03±25.28）U/mg比较也下降,差异均有统计学意义（P〈0.05）;实验组左睾丸内的SDH活性（1.22±0.41）U/mg与对照组同侧（1.74±0.43）U/mg比较下降,差异有统计学意义（P〈0.05）,与实验组右睾丸（1.62±0.56）U/mg比较下降,差异无统计学意义（P〉0.05）;实验组左睾丸内PCNA（669.10±205.39）mU/ml与对照组同侧（776.81±231.72）mU/ml比较下降,和实验组右睾丸（800.81±172.02）mU/ml比较也下降.差异均无统计学意义（P〉0.05）.结论 改进型ELV致睾丸酶活性降底和PCNA的变化,这些变化可能是影响生育能力的因素之一.

Objective To evaluate the effects of improved left varicocele （ELV） on testis enzymes and proliferating cell nuclear antigen （PCNA） in rats. Methods ELV models of SD rats were established by the improved method in 21 SD rats, and another 15 were included in a sham operation group as controls. At the 3rd month, the activity of LDH, SDH and G6PDH was measured by colorimetry. The concentration of the PCNA was determined by enzyme-linked immunosorbent assay （ ELISA）. Results The activity of LDH and G6PDH （8. 17 ± 3. 47）, （34. 00 ± 16. 29） U/mg in left testes of experimental group was reduced remarkably as compared with that in right testes （12.69 ±3.97）, （78.03 ± 25. 28） U/mg and control group （11. 98 ± 2. 26） , （54. 88 ± 20. 87） U/mg （P 〈 0. 05）. The activity of SDH was reducd remarkably in left testes of experimental group （ 1. 22 ±0. 41 ） U/mg as compared with that in control group （1.74 ± 0.43） U/mg （P 〈 0.05）, but there was no significant difference between the left and right testes （ 1.62 ±0. 56） U/mg in experimental group. The concentration of PCNA was reduced in left testes of experimental group （669. 10 ±205. 39） mU/ml as compared with that in right testes （800. 81 ± 172. 02） mU/ml and control group （776. 81 ± 231. 72） mU/ml, but there was no significant difference （P 〉 0. 05）. Conclusion The reduction of LDH, SDH and G6PDH and the changes of PCNA in improved ELV may play an important role in the development of subfertility.