干旱胁迫下氯化胆碱对小麦幼苗叶片中叶绿素含量和荧光特性伤害的缓解作用

Alleviation of choline chloride on damage of chlorophyll and chlorophyll fluorescence parameters in wheat seedlings under drought stress

实验以淮麦21(Triticumaestivum L.)为材料,于小麦幼苗长至两叶一心期模拟干旱处理,并用不同浓度(0,100,200,300,400,500 mg/L)的氯化胆碱(CC)水溶液喷施小麦幼苗叶片,测定处理后的小麦叶绿素含量以及叶绿素荧光动力学参数。结果表明:用一定浓度氯化胆碱(CC)水溶液喷施小麦叶面,能明显提高干旱胁迫下小麦叶片中叶绿素含量,300 mg/L浓度的氯化胆碱处理时叶绿素a和叶绿素总量分别比对照提高83.46%和81.74%。且仍能维持较高的Fo′(光适应后最小荧光)、Fm′(光适应后的最大荧光)、Fv′(可变荧光)、Fv′/Fm′(最大光化学效率)、qP(光化学猝灭系数)、ETR(电子传递速率)和ΦPSⅡ(实际的电子传递的量子效率)等荧光参数。说明氯化胆碱能够缓解干旱胁迫下对叶绿素以及叶绿素荧光特性的伤害。

The effects of choline chloride（CC） on chlorophyll content and chlorophyll fluorescence parameters（Fo,Fm,Fv,Fo′,Fm′,Fv′,qP,ETR and NPQ） on wheat（Triticum aestivum L.Huaimai 21） seedlings were studied after treatments with different concentrations（0,100,200,300,400 and 500 mg/L） under drought stress.The results showed that the content of chlorophyll a and content of chlorophyll were obviously increased by 83.46% and 81.74% respectively after spraying choline chloride（CC） with 300 mg/L concentration in wheat seedlings under drought stress.Also the chlorophyll fluorescence parameters（Fo′,Fm′,Fv′,Fv′/Fm′,qP,ETR and ΦPSⅡ） were maintained higher than CK.It was suggested that there would be an alleviation of choline chloride on damage of chlorophyll and chlorophyll fluorescence parameters in wheat seedlings under drought stress.