摘要
用０．７６％的ＥＤＴＡ（ｅｔｈｙｌｅｎｅｄｉａｍｉｎｅｔｅｔｒａｃｅｔｉｃａｃｉｄ）分离剥取输卵管上皮细胞是一种好的试剂，１０％胎牛血清（ＦＣＳ）ＴＣＭ－１９９（Ｅａｒｌ’ｓｓａｌｔｓ）培养其上皮细胞，５～６ｄ即可生长成单层，寿命可延至３～４周，共同培养从第７天开始，其囊胚发育率显著高于与颗粒细胞共同培养。
EDTA is good reagent to seperate bovine epitheliumcells.Epithelium cells were cultured by TCM-199 with 10% FCS;after 5～6 days monolyer was formed,and could survive 3～4 weeks.Coculture began on day 7.Blasteyst development rate wassignificantly higher than that of granulose cells.
出处
《黑龙江畜牧兽医》
CAS
北大核心
1999年第2期5-6,共2页
Heilongjiang Animal Science And veterinary Medicine
