应用大鼠前额叶皮层多通道局部场电位的同步模式研究丙泊酚麻醉程度

Synchronous pattern of multi-channel local field potentials in rats anesthesized by propofol

目的 研究在清醒、丙泊酚麻醉过程中、翻正反射恢复后3种状态下,大鼠前额叶皮层多通道局部场电位（LFPs）的同步模式,作为界定麻醉程度的客观指标.方法 利用成年SD大鼠5只,在大鼠前额叶皮层植入16通道微电极阵列10 d后,应用Cerebus多通道信号采集分析系统,记录大鼠在清醒、丙泊酚麻醉过程中、翻正反射恢复后3种状态下前额叶皮层的16通道信号.丙泊酚麻醉按0.1 mg/（kg体重·min）的剂量,恒速静脉输注.对记录的原始信号进行预处理,获取LFPs,应用Hilbert变换计算LFPs相位系列.选择参考通道,分别计算3种状态下4s时间段内其它15通道LFPs相位与参考通道LFPs相位的相关动态值,计算窗口200 ms,窗口移动步长50 ms.结果 大鼠在清醒状态下LFPs相位的相关动态值比在丙泊酚麻醉状态下小（P〈0.05）;恢复翻正反射后的LFPs相位相关动态值比清醒状态大（P〈0.05）,比麻醉状态下小（P〈0.05）.结论 大鼠在清醒状态下,其前额叶皮层LFPs相位未呈现同步性;在丙泊酚麻醉状态下,呈现高度的同步性;在麻醉复苏期间,同步性在2者之间.多通道LFPs相位的同步程度可以界定不同的麻醉程度.

Objective To study the phase coherence mode of multi-channel local field potentials （LFPs） of rats cortex under waking, propofol anesthetized and recovery of righting reflex situations and to apply it to estimate the anesthetic degree. Methods Five male Sprague-Dawley rats were selected and 16-channel micro-electrode matrix were implanted in the prefrontal cortex of the rats. Ten days later, 16-channel electric signals were recorded from waking rats using Cerebus 128 Data Acquisition System. Then the rats were anesthetized with propofol intravenously through the tail vein, with the induction dose of propofol of 10 mg/kg and anesthesia maintenance dose of put and the rat regained consciousness, 16-channel electric signals from anaesthetic rats were recorded. After preprocessing, phase coherence of LFPs was calculated among the data from these three situations. Phase coherences were calculated in each window between each signal channel LFP and reference channel LFP with 200-ms multitaper window sliding from the beginning. Time-varying phase coherence dynamic distribution was available between each channel LFP and reference channel LFP with 25-ms overlapping. Results The results shows that the phase coherence average number in waking situation is less than the number in anesthesia （P〈0.05）; the phase coherence average number in recovery situation is less than that of anesthesia （P〈0.05）, it is larger than the number in anesthetic situation on the contrary （P〈0.05）. Conclusion The result indicates that the no synchronization of the LFPs during the waking-up time was found. High synchronization of the LFPs during the anesthetic period was found, while the degree of synchronization of the LFPs during the recovery time was between that of waking-up time period and anesthetic period. It can be applied to identify anesthetic degree of propofol.