抑制骨架蛋白对正常大鼠肾细胞力学性能的影响

Effect of inhibiting cytoskeleton on the mechanical properties of normal rat kidney cells

目的确定正常大鼠肾细胞内骨架蛋白的变化对细胞力学性能的影响。方法通过微管吸吮技术对正常大鼠肾细胞用细胞松弛素D、秋水仙素和blebbistatin进行处理,然后分析细胞的杨氏模量和黏弹性的变化。结果经过骨架蛋白抑制剂的处理,细胞的弹性模量表现出显著的下降;与对照组相比,秋水仙素处理后细胞的黏弹性没有显著变化,而细胞松弛素D和blebbistatin处理后细胞的黏弹性显著降低。结论Blebbistatin对正常大鼠肾细胞力学特性的影响不依赖于细胞骨架的改变;抑制肌动蛋白聚合或抑制肌球蛋白IIATP酶的活性能够大幅降低细胞杨氏模量和黏弹性,微管蛋白是否正常聚合与细胞杨氏模量相关而对细胞黏弹性没有显著影响。

Objective To determine whether the alteration of cytoskeleton interfere with the mechanical properties of normal rat kidney cells （NRKCs）.Method NRKCs were treated with cytochalasin D,colchicine and blebbistatin using micropipette aspiration technique,and the changes in Young＇s modulus and viscoelasticity of NRKCs were assessed. Results The Young＇s modulus of is significantly decreased after treated with all cytoskeleton inhibitors. Compared to the control,the viscoelasticity is significantly decreased in NRKCs treated with cytochalasin D and blebbistatin,but in not in colchicines-treated cells. Conclusions The effect of blebbistatin on NRKCs mechanical properties is independent of cytoskeleton. Inhibition of assembling of actin meshwork or the activity of myosin II ATPase will reduce cell＇s Young＇s modulus and viscoelasticity. In addition,microtubule assembling is correlated with Young＇s modulus rather than viscoelasticity.