原发性肝癌DSA表现特点与癌组织血管内皮细胞生长因子表达相关性研究

The DSA characteristics and the expression of VEGF of primary hepatocellular carcinoma: a correlative study

目的探讨肝细胞性肝癌(HCC)DSA表现特点与癌组织血管内皮生长因子(VEGF)表达的关系。方法选择54例经病理证实原发性HCC患者,根据其在DSA的表现特点,如肿瘤大小及多少、病灶边缘是否清晰、肿瘤血供情况(包括肿瘤血管的丰富度及是否有动静脉瘘等)等特点进行分析;用ElivisionTMplus法对HCC组织切片进行VEGF定性检测,根据染色程度及阳性细胞的百分比进行综合分析;同时进行HE染色,用Edmmond法进行病理分级,本组把Ⅰ、Ⅱ级划为高分化组,Ⅲ、Ⅳ级划为低分化组。对结果进行统计学分析,采用SPSS13.0统计软件进行数据处理。结果肝癌组织的VEGF表达与癌组织血供特点、病灶数目多少、轮廓完整性及肿瘤分化程度相关(P<0.05或P<0.0167),与瘤体大小无关(P>0.05)。结论原发性肝癌DSA某些表现特征与肝癌组织的VEGF含量有一定相关性,通过观测DSA图像特征能提高对肝癌侵袭和转移评估的能力,给临床个性化治疗和判断预后提供参考。

Objective To discuss the DSA characteristics of primary hepatocellular carcinoma （HCC） and to investigate the relationship between the DSA findings and the expression of tumor’s vascular endothelial growth factor （VEGF）. Methods Fifty-four patients with pathologically-proved primary HCC were enrolled in this study. The DSA findings, including tumor’s size, lesion’s number, lesion’s margin, the degree of blood supply, the presence or absence of artreiovnous shunting （AVS）, etc. were observed and analyzed. The qualitative test of VEGF of HCC tissue was made by using Elivision TMplus method, and based on the stain degree and the percentage of the positive cells a comprehensive analyse was carried out. With HE stain the pathologic classification was conducted by using Edmmond method. In this study, grade I and II were regarded as well-differentiated group and grade III and IV were regarded as poorly-differentiated group. The data were processed by software SPSSS13.0 and the results were statistically analyzed. Results The expression degree of VEGF in HCC tissue was related to the blood supply pattern, the lesion’s number, the integrity of the tumor’s border and the degree of differentiation （P〈0.05）, while it showed no correlation with the tumor’s size （P 〉0.05）. Conclusion Some DSA manifestations of primary HCC bear a certain relationship to the VEGF content in the tumor tissue. Through observing the DSA characteristics, the assessment of the invasion and metastasis of HCC can be well made, which will afford the clinical physicians a useful reference in making individualized therapeutic schedule and in estimating the prognosis.