转化生长因子β1、结蛋白及CD34在大鼠阴茎海绵体组织中的表达

Expressions of transforming growth factor-β1,Desmin and CD34 in the penile corpus cavernosum of rats

目的:检测转化生长因子β1(TGF-β1)、结蛋白(Desmin)及CD34在不同月龄组SD大鼠阴茎海绵体组织中的表达情况。方法:随机选取2、5、20月龄不同窝别SD大鼠各10只分别作为幼龄组、壮龄组和老龄组,乙醚麻醉下取阴茎海绵体组织,分别用RT-PCR和免疫组化检测TGF-β1、Desmin及CD34mRNA及蛋白的表达。结果:RT-PCR结果显示,阴茎海绵体组织中有TGF-β1、Desmin及CD34mRNA的表达,且3者表达量于组间两两比较均有统计学差异(P<0.01)。TGF-β1蛋白主要表达于海绵体小梁及动脉血管周围,胞膜、胞质染色;Desmin蛋白为肌性组织染色,以胞膜、胞质为主;CD34蛋白表达以血管及海绵窦内皮为主。TGF-β1mRNA的表达与月龄呈明显正相关(r=0.944,P<0.01),Desmin及CD34mRNA的表达与月龄呈明显负相关(r=-0.947,P<0.01;r=-0.934,P<0.01),且Desmin、CD34mRNA的表达均与TGF-β1mRNA的表达有明显的相关性(r=-0.888,P<0.01;r=-0.887,P<0.01)。结论:随着月龄的增长,阴茎海绵体组织中TGF-β1的表达明显增加,Desmin、CD34的表达明显减少;TGF-β1的表达与Desmin、CD34的表达均呈显著负相关;TGF-β1是ED的重要影响因子。

Objective：To detect the expressions of transforming growth factor-β1 （TGF-β1）,Desmin and CD34 in the penile corpus cavernosum of SD rats in different age groups.Methods：We randomly selected 10 SD rats in each of the 2-,5-and 20-month age groups,harvested their penile corpus cavernosum tissues under ether anesthesia,and detected the mRNA and protein expressions of TGF-β1,Desmin and CD34 by RT-PCR and immunohistochemistry.Results：The results of RT-PCR showed the mRNA expressions of TGF-β1,Desmin and CD34 in the corpus cavernosum tissues,with significant differences between every two groups （P 0.01）.The TGF-β1 protein was mainly expressed in the trabeculae and around the arteries of the corpus cavernosum for membrane and cytoplasm staining,the Desmin protein mainly in the membrane and cytoplasm for muscle tissue staining;and the CD34 protein mainly in the vascular and sinusoidal endothelia.The mRNA expression of TGF-β1 was correlated positively （r = 0.944,P 0.01） while those of Desmin and CD34 negatively with the age of the rats （r =-0.947,P 0.01;r =-0.934,P 0.01）.And the mRNA expressions of both Desmin and CD34 had a significant correlation with that of TGF-β1 （r =-0.888,P 0.01;r =-0.887,P 0.01）.Conclusion：With the increase of age,the expression of TGF-β1 is significantly up-regulated,while those of Desmin and CD34 significantly down-regilated in the corpus cavernosum tissues,and it is negatively correlated with the latter two.TGF-β1 is an important influencing factor on ED.