Mdr-1基因siRNA表达载体联合磁性纳米Fe_3O_4对转染率的影响

Influence of the combination of Mdr-1shRNA expression vetor and magnetic fe3o4 nanoparticle on transfection efficiency

目的探讨磁性纳米Fe3O4颗粒(Fe3O4-MNPs)对Mdr-1基因siRNA表达载体转染白血病耐药细胞株K562/A02细胞转染效率的影响。方法构建靶向Mdr-1基因的PGCsilencer-U6-neo-GFPsiRNA表达载体(PGY1-1),分别与10、15、20μg/mlFe3O4-MNP置于37℃、5%CO2条件下共孵育,荧光显微镜下计数48h细胞的转染效率。结果 PGY1-1对K562/A02细胞转染率未见明显变化。结论 PGY1-1对K562/A02细胞的转染效率不能通过共孵育的方法得到提高。

Objective To investigate the influence of magnetic nanoparticle of Fe3O4[MNP（Fe3O4）] on transfection efficiency in cell lines K562/A02.Methods Gene Mdr-1-targeting short hairpin RNA （shRNA） eukaryotic expression vector （PGY1-1） was constructed,which was coincubated with MNP（Fe3O4） 10,15,20 μg /ml,respectiveiy,at 37℃ in CO2 incubator for 48 hours.The transfection efficiency was counted with fluorescence microscope.Results The transfection efficiency of K562/A02 was not elevated.Conclusion The transfection effect of eukaryotic expression vector PGY1-1 on K562/A02 can not be enhanced by MNP（Fe3O4） coincubation.