摘要
目的探讨磁性纳米Fe3O4颗粒(Fe3O4-MNPs)对Mdr-1基因siRNA表达载体转染白血病耐药细胞株K562/A02细胞转染效率的影响。方法构建靶向Mdr-1基因的PGCsilencer-U6-neo-GFPsiRNA表达载体(PGY1-1),分别与10、15、20μg/mlFe3O4-MNP置于37℃、5%CO2条件下共孵育,荧光显微镜下计数48h细胞的转染效率。结果 PGY1-1对K562/A02细胞转染率未见明显变化。结论 PGY1-1对K562/A02细胞的转染效率不能通过共孵育的方法得到提高。
Objective To investigate the influence of magnetic nanoparticle of Fe3O4[MNP(Fe3O4)] on transfection efficiency in cell lines K562/A02.Methods Gene Mdr-1-targeting short hairpin RNA (shRNA) eukaryotic expression vector (PGY1-1) was constructed,which was coincubated with MNP(Fe3O4) 10,15,20 μg /ml,respectiveiy,at 37℃ in CO2 incubator for 48 hours.The transfection efficiency was counted with fluorescence microscope.Results The transfection efficiency of K562/A02 was not elevated.Conclusion The transfection effect of eukaryotic expression vector PGY1-1 on K562/A02 can not be enhanced by MNP(Fe3O4) coincubation.
出处
《江苏医药》
CAS
CSCD
北大核心
2010年第10期1170-1171,F0003,共3页
Jiangsu Medical Journal
基金
国家自然科学基金(39970832
30740062)
高等学校博士学科点专项基金(20070286042)