OX40-OX40L在人单核细胞和冠脉斑块中的共同表达

Coexpression of OX40 and OX40 ligand on human monocytes and in coronary atherosclerotic plaque lesions

目的探讨细胞刺激分子OX40及其配体OX40L在人单核细胞及冠状动脉粥样硬化斑块(冠脉斑块)中的表达。方法采用荧光技术、流式细胞术检测OX40、OX40L在单核细胞表面的表达,免疫组化方法检测在冠脉斑块中的表达。结果 OX40、OX40L在人单核细胞能连续表达,且细胞因子IL-1β、IL-6、TNF-α和INF-γ能明显刺激其表达;冠脉斑块中能共同表达OX40L(肩部和底部)和OX40(广泛),而动脉壁其它部分不表达。结论人单核细胞表面及冠脉斑块中能共同高表达OX40和OX40L。

Objective To investigate the coexpression of OX40 and OX40 and its ligand （OX40L） on human monocytes and in coronary atherosclerotic plaque lesions.Methods The expressions of OX40 and OX40L on human monocytes were measured by fluorescence microscope and flow cytometry,respectively.The expressions OX40 and OX40L in atheroma plaques were detected by immunohistochemistry.Results Cultured human monocytes continuously coexpressed OX40 and OX40L on the surface of the cells.Stimulation with interleukin（IL）-1β,IL-6,tumor necrosis factor（TNF）-α and interferon-Υ increased the levels of OX40 and OX40L on monocytes.Human coronary atherosclerotic lesions showed the coexpressions of immunoreactive OX40 and OX40L.OX40L mainly expressed on the shoulder and base of the plaque,but OX40 was widespreadly expressed in the plaque.There were no expressions of OX40 and OX40L in nonatherosclerotic human arteries.ConclusionOX40 and OX40L could coexpress on the surface of human monocytes and in human atherosclerotic plaque lesions.