蛋白基因产物9.5与神经丝蛋白标记瘢痕神经效果的比较

The comparison of scar nerve fiber staining by protein gene product 9.5 and neurofilament protein

目的比较蛋白基因产物9.5(PGP9.5)与神经丝蛋白(NFP)对瘢痕组织内神经纤维的染色效果。方法采取瘢痕组织标本25份,由两实验者分别采用免疫荧光技术以PGP9.5、NFP作为神经标记物进行实验,测量免疫阳性神经纤维灰度并计算神经容积分数NVF,对免疫阳性神经纤维进行半定量分析;比较实验者、标记物对染色以及两标记物染色阳性率的差异。结果⑴实验者对实验数据没有造成显著差异(F=0.542,P>0.05);⑵PGP9.5组的灰度值51.55±41.47,显著低于NFP组的81.38±32.1,(P<0.05);⑶PGP9.5组NVF值为0.57±0.38,显著低于NFP组的NVF0.82±0.28,(P<0.05);⑷PGP9.5染色阳性率也低于NFP组(P<0.05)。结论 PGP9.5标记人瘢痕神经纤维的效果不如NFP。

Objective To compare the effects on scar nerve fiber staining by PGP9.5 and NFP.Methods 25 scar samples at the proliferative or maturation stages were harvested.Two different researchers performed test respectively.Scar nerve fibers were labeled with PGP9.5 or NFP by immunofluorescent technique.Gray values were determined and nerve fiber volume fractions（NVF） were calculated adopting semi-quantitation.Positive rate of different markers were determined.Results ⑴There was no significant difference between researchers（F=0.542,P0.05）.⑵Gray value of PGP9.5 positive nerve fibers in scar were 51.55±41.47,which were lower than that of NFP（81.38±32.1,P0.05）;⑶ NVF of PGP9.5 positive nerve fibers in scar were 0.57±0.38,lower than that of NFP 0.82±0.28,（P0.05）.⑷PGP9.5 positive rate was significant lower than that of NFP.Conclusions PGP9.5 is less effective in labeling scar nerve fibers than NFP.