刺参糖胺聚糖对HBV转基因小鼠肝组织HBV相关抗原表达的影响

Effects of glycosaminoglycan extracted from Stichopus japonicus Selenka on the expression of HBV-associated antigens in the liver of HBV transgenic mice

目的:研究刺参糖胺聚糖在动物活体内的抗HBV作用.方法:开放性动物实验.HBV转基因小鼠30只,按性别随机分为生理盐水对照组(每天给予等容积的生理盐水)、刺参糖胺聚糖低(30mg/kg)、中(40mg/kg)、高(50mg/kg)三个剂量组及干扰素(45U/kg)对照组,每组雌雄各半,分笼饲养.给药方式为腹腔注射,每日1次,连续用药30d.给药结束后处死小鼠,剥离其肝脏组织.免疫组织化学染色法及综合评分法观察并半定量分析小鼠肝脏组织HBsAg,HBcAg的表达.结果:与生理盐水对照组比较,刺参糖胺聚糖低、中、高剂量对小鼠肝脏HBsAg,HBcAg的表达均有抑制作用(4.93±1.43,3.57±1.02,3.27±0.64vs6.97±0.75;5.73±1.62,4.27±1.03,4.27±1.03vs7.23±0.53,P<0.01或0.05),但其抑制作用低于干扰素对照组(P<0.01或0.05),并呈现一定的量效反应关系.结论:刺参糖胺聚糖具有一定的体内抗HBV作用.

AIM：To study the potential anti-hepatitis B virus （HBV） effects of glycosaminoglycan extracted from Stichopus japonicus Selenka （SJ-GAG） in vivo.METHODS：Thirty HBV transgenic mice were divided into five groups：saline group,interferon （IFN） group,and low-,medium-and high-dose SJ-GAG group,which were intraperitoneally injected with normal saline,IFN-α2b （45 U/kg）,and SJ-GAG （30,40 and 50 mg/kg,respectively） once daily for 30 d,respectively.After treatment,all mice were killed to take liver samples for analysis of the expression of HBsAg and HBcAg by immunohistochemistry.RESULTS：Compared to the saline group,the expression of HBsAg and HBcAg in the liver of HBV transgenic mice was dose-dependently inhibited in all the SJ-GAG groups （4.93 ± 1.43,3.57 ± 1.02,3.27 ± 0.64 vs 6.97 ± 0.75;5.73 ± 1.62,4.27 ± 1.03,4.27 ± 1.03 vs 7.23 ± 0.53;P 〈0.01 or 0.05）.However,SJ-GAG has lower inhibitory effects on the expression of HBsAg and HbcAg than IFN （P〈 0.01 or 0.05）.CONCLUSION：SJ-GAG has anti-HBV activity in vivo.