食管鳞癌clusterin表达及其临床意义

Clinical significance of clusterin expression in esophageal squamous cell carcinoma

目的:探讨食管鳞癌组织clusterin表达和单纯手术治疗前后外周血clusterin含量变化与食管鳞癌临床参数的相关性.方法:RT-PCR分析5对食管鳞癌及其远端切缘食管上皮中clusterin基因mRNA表达水平;ELISA分析41例单纯手术治疗食管鳞癌患者治疗前后的血清clusterin蛋白表达水平及其与临床特征的相关性.结果:与正常食管上皮相比食管鳞癌组织中clusterin基因表达显著下降或缺失.食管鳞癌患者术前血清中clusterin含量明显低于术后血清含量(3.23mg/Lvs25.71mg/L,P<0.0001).食管鳞癌患者术前血清clusterin含量与肿瘤大小相关(P<0.01),而与年龄、性别、分化程度、肿瘤分期、淋巴结转移,以及总蛋白、白蛋白、血糖、血脂和总胆红素浓度无关(r=-0.1334,-0.1602,0.2413,0.0389,-0.2882,均P>0.05).结论:Clusterin基因在食管鳞癌组织中表达明显下调或缺失,血清clusterin含量明显降低,提示在食管鳞状细胞癌中clusterin基因可能具有潜在的抑癌基因作用,动态检测外周血clusterin含量有助于判断食管癌进展.

AIM：To investigate the expression of clusterin mRNA in esophageal squamous cell carcinoma （ESCC）,measure preoperative and postoperative serum clusterin protein levels in ESCC patients,and evaluate their correlations with clinicopathological parameters in ESCC.METHODS：The expression of full-length clusterin mRNA in ESCC tissue was detected by semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）.Serum clusterin level was measured by enzyme-linked immunosorbent assay （ELISA）.RESULTS：The expression of clusterin mRNA was significantly down-regulated in ESCC tissue compared with matched tumor-adjacent noncancerous tissue.The median level of serum clusterin in postoperative ESCC patients was significantly higher than that in preoperative patients （25.71 mg/L vs 3.23 mg/L,P〈 0.0001）.The level of serum clusterin is correlated with tumor size,but not with age,gender,tumor differentiation,tumor grade,lymph node metastasis and biochemical parameters.CONCLUSION：The expression of clusterin mRNA is down-regulated in ESCC.Serum clusterin level decreases in ESCC patients.Clusterin might be a potential tumor suppressor gene in ESCC.Dynamic measurement of serum clusterin level might aid to evaluate the progression of ESCC.