抗肝癌免疫纳米颗粒的制备及其对肝癌细胞增殖的影响

Preparation of hepatocellular carcinoma-targeted immunonanoparticles and investigation of their effects on the proliferation of hepatocellular carcinoma cells

目的:制备抗肝癌单链抗体二聚体高分子免疫纳米颗粒,观察其对肝癌细胞增殖的影响.方法:采用离子交联的方法,以壳聚糖水溶性衍生物多糖为基材,将已获得的抗肝癌单链抗体二聚体BDM制备成免疫纳米颗粒,检测纳米颗粒的表征、包封率及载药量,并通过MTT法观察免疫纳米颗粒对肝癌细胞株增殖的影响.结果:制备粒径为100-200nm的抗肝癌单链抗体二聚体高分子纳米颗粒,最佳包封率为53%,载药量为75μg/mg抗体二聚体,抗肝癌单链抗体二聚体高分子纳米颗粒显示较好的抗肿瘤作用,其对肝癌细胞的抑制率为34%左右,且有浓度依赖性.结论:成功制备了抗肝癌单链抗体二聚体高分子纳米颗粒,初步应用具有抑瘤性,为下一步开展体内肝癌的放射免疫诊断和靶向治疗奠定了基础.

AIM：To prepare polyelectrolyte nanoparticles loaded with humanized single-chain Fv dimers for hepatocellular carcinoma （BDM diabody）,and to investigate their effects on the proliferation of hepatocellular carcinoma （HCC） cells.METHODS：By means of infrared spectroscopy and transmission electron microscopy,the physicochemical characteristics of polyelectro-lyte nanoparticles were characterized,including particle size and morphology,the concentration of protein loaded,and the protein entrapment efficiency of the nanoparticles. The effects of BDM-loading nanoparticles on the proliferation of HCC cells were determined by methyl thiazol tetrazolium （MTT） assay. RESULTS：The particle size ranged from 100 to 200 nm. The concentration of protein loaded was 75 μg/mg. The protein entrapment efficiency was 53%. BDM-loading nanoparticles could sig-nif icantly inhibit the proliferation of HCC cells.CONCLUSION：HCC-targeted nanopaticles are successfully prepared,which provides a basis for future targeted diagnosis and therapy of HCC.