食管鳞癌患者血清RUNX3基因甲基化检测及临床意义

Analysis of RUNX3 promoter hypermethylation in serum DNA of esophageal squamous cell carcinoma patients

目的检测食管鳞癌患者血清中RUNX3基因启动子区域甲基化状态,探讨用于食管鳞癌早期诊断和预后评估的临床意义。方法留取70例食管鳞癌,20例食管良性病变及10例健康志愿者血清标本,甲基化特异性聚合酶链反应(MSP)分析RUNX3基因启动子区域甲基化情况,并分析其与临床病理参数之间的相关性。结果 70例食管鳞癌患者血清RUNX3基因启动子区域异常甲基化36例,检出率为51.4%,20例食管良性病变患者中有2例为不完全甲基化(10%),而10例健康志愿者中检出率为0,差异有统计学意义(P<0.001);RUNX3基因启动子甲基化与患者临床分期和淋巴结转移相关。结论 RUNX3基因启动子甲基化在食管鳞癌患者血清中有着较高的检出率,可望成为食管鳞癌早期诊断和预后评估的分子标志物。

Objective To detect the promoter methylation status of RUNX3 gene in serum DNA of esophageal squamous cell carcinoma （ESCC） patients and evaluate its correlation with clinicopathological features. Methods Serum DNA was extracted from peripheral blood of 70 ESCC patients and 30 controls of benign esophageal diseases or healthy donors. Promoter methylation status of RUNX3 gene was determined by methylation-specific polymerase chain reaction （ MSP）, and the correla- tion between methylation profiles and clinieopathological parameters was statistically analyzed. Results Aberrant methylation of RUNX3 gene was detected in 36 of 70 （51.4% ） ESCC patients, the frequency was significantly higher than that in benign esophgeal disease （2/20, 10% ） and healthy controls （0/10） （P 〈0. 001）. RUNX3 hypermethylation status was found to be correlated with advance stage and lymph metastasis in ESCC patients. Conclusion Frequent hypermethylation of RUNX3 promoter exists in ESCC, suggesting it may be a promising biomarker for the early diagnosis and prognosis assessment.