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不同真空处理时间对辣根过氧化物酶二级结构的影响 被引量：2

Time effect of vacuum treatment on secondary structure of horse radish peroxide
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摘要 辣根过氧化物酶经真空处理不同时间,利用圆二色光谱研究不同真空处理时间对辣根过氧化物酶二级结构的影响。结果表明：在3~15 min时间内,不同真空处理时间对辣根过氧化物酶的α-螺旋、β-折叠、β-转角及无规卷曲相对含量产生不同程度的影响,但是影响程度较小,表明辣根过氧化物酶二级结构相对力学作用具有较高的稳定性。 The time effects of different vacuum treatment on the secondary structure of horse radish peroxide（HRP） were investigated experimentally via the circular dichroism（CD） spectra.The results revealed that the different vacuum treating time（3～15min） affects the related contents of α-helix,β-folding,β-turn and random coiling polymer differently and unobviously,ie.,the secondary structure of HRP is highly stable in view of mechanics.
作者 许强 王苗
机构地区 宝鸡文理学院物理与信息技术系 宝鸡文理学院图书馆
出处 《真空》 CAS 北大核心 2010年第3期75-77,共3页 Vacuum
基金 陕西省自然科学基础研究计划项目(2007A18) 宝鸡文理学院重点项目(ZK09130 ZK09131 ZK09132)
关键词 真空处理 辣根过氧化物酶 圆二色谱 二级结构 vacuum treatment HRP cicrulary dichroitic spectrum secondary structure
分类号 Q689 [生物学—生物物理学] Q946.5 [生物学—植物学]
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参考文献12

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共引文献219

同被引文献46

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真空
2010年 第3期

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