摘要
目的 探讨贲门癌患者的免疫功能异常及其临床意义.方法 采用流式细胞术（FCM）和酶联免疫吸附（ELISA）方法分别检测56例贲门癌患者、15名健康人外周血中CD4^＋CD25^hiCD127^lowc调节性T细胞（Treg细胞）和血清IL-10、TGF-β1水平,结合临床资料进行分析.结果 56例贲门癌患者外周血中CD4^＋CD25^hiCD127lowTreg细胞占CD4^＋淋巴细胞的比例为（5.73±1.56）%,与健康对照组的（4.45±1.06）%相比,差异有统计学意义（P〈0.01）;血清中IL-10和TGF-β1含量均明显高于健康对照（P〈0.05）.贲门癌患者外周血CD4^＋CD25^hiCD127low Treg细胞水平与血清IL-10和TGF-β1含量呈正相关.贲门癌患者外周血CD4^＋CD25^hiCD127low Treg细胞数量与患者临床分期、淋巴结转移有关.结论 贲门癌患者外周血中CD4^＋CD25^hiCD127low Treg细胞表达增高,与临床分期有关,提示Treg水平异常与贲门癌的发生发展密切相关.
Objective To analyse the dysfunction of immunity and clinical significance in patients with cardiac cancer. Methods The level of CD4^＋CD25^hiCD127^low cells were detected by flow cytometry （FCM）, and serum IL-10 and TGF-β1 levels were determined by enzyme linked immunosorbent assay （ELISA） kit in 56 patients with cardiac cancer.15 healthy volunteers were tested as normal controls. The clinical data of each patient were collected and analyzed. Results There was a significantly higher percentage of CD4^＋CD25^hiCD127^low cells in patients with cardiac cancer（5.73±1.56）% than that（4.45±1.06）% of healthy volunteers （P 〈0.01）. The IL-10 and TGF-β1 levels in the serum of patients with cardiac cancer were also significantly higher than that of healthy volunteers（P 〈0.05）. There was a positive correlation between levels of IL-10, TGF-β1 and the levels of CD4^＋CD25^hiCD127^lowcells. The number of CD4^＋CD25^hiCD127^low regulatory T cells in the peripheral blood of cardiac cancer patients were significantly correlated with clinical stages and metastasis lymph node. Conclusion The CD4^＋CD25^hiCD127^low cells in the peripheral blood of cardiac cancer patients is significantly increased in comparison with that in healthy volunteers, and was also correlated with different stages. The abnormal levels of CD4^＋CD25^hiCD127^low cells may be related to tumor progression in patients with cardiac eancer.
出处
《肿瘤研究与临床》
CAS
2010年第5期303-305,共3页
Cancer Research and Clinic
