诺氟沙星-铜(Ⅱ)-TBZ/HPB配合物的合成、抗菌活性及与DNA作用

Synthesis,Antibacterial Activities and DNA Binding Properties of Two New Cu(Ⅱ) Complexes with Norfloxacin and TBZ/HPB

合成了2个新的配合物:[Cu(NFA)(TBZ)(H2O)](NO3).(H2O)0.5(1)和[Cu(NFA)(HPB)(H2O)]NO3(2)[NFA=1-乙基-6-氟-1,4-二氢-4-氧代-7-(哌嗪-1-基)-3-喹啉羧酸(诺氟沙星),TBZ=2-(噻唑-4′-基)苯并咪唑,HPB=2-(吡啶-2-基)-苯并咪唑]。用元素分析、摩尔电导率、红外光谱和紫外可见光谱等方法对配合物进行了表征;通过二倍稀释法研究了配合物对枯草杆菌、大肠杆菌和沙门氏菌的抑制作用;用电子吸收光谱、荧光光谱和粘度测定等方法研究了配合物与CT-DNA的作用。结果表明,这2种配合物均具有良好的抗菌活性,能以插入方式与CT-DNA结合,且抗菌活性与DNA结合能力大小一致:配合物2>1。

Two new complexes：[Cu（NFA）（TBZ）（H2O）]（NO3）.（H2O）0.5（1） and [Cu（NFA）（HPB）（H2O）]NO3（2）（NFA = 1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-（1-piperazinyl）-quinoline-3-carboxylic acid（Norfloxacin）,TBZ = 2-（4＇-thiazolyl） benzimidazole,and HPB = 2-（2-pyridyl） benzimidazole） were synthesized and characterized by elemental analysis,molar conductivity,IR and UV-Vis spectra.The complexes were assayed against Escherichia coli,Salmonella,and Bacillus subtilis by the doubling dilution method,and the interaction between the complexes and Calf thymus DNA was investigated by electronic absorption,fluorescence,and viscosity studies.The results showed that the complexes have good antibacterial activities and could bind to DNA via an intercalative mode,giving an order：complex 2〉1.