低剂量粒细胞集落刺激因子动员健康供者造血干细胞的研究

Peripheral stem cell mobilization with low-does of G-CSF in healthy donors

目的 探讨低剂量（5μg·kg^-1·d^-1）粒细胞集落刺激因子（G-CSF）对健康供者CD34^＋细胞动员的效果及造血干细胞最佳采集时间.方法 对2006年2月至2009年4月108例健康供者给予G-CSF 5μg·kg^-1·d^-1，在动员后的第4天至第6天收集标本,检测相应时间点的外周血白细胞（WBC）计数、采集物单个核细胞（MNC）计数、采集物中CD34^＋细胞的比例、粒-巨噬细胞集落形成单位（CFU-GM）培养.结果 动员后采集物中第4天至第6天CD34^＋细胞比例分别为（0.71±0.08）%、（1.09±0.09）%、（057±0.08）%.第4天至第6天CFU-GM产率分别为：（93.33±44.51）/105MNC、（124.61±57.85）/105MNC和（80.25±49.24）/105MNC.CD34＋细胞比例和CFU-GM产率均在第5天达到峰值（P〈0.05）,第4天次之（P〈0.05）.第6天再次之（P〈0.05）.动员后采集物中第4天至第6天CD34^＋细胞量分别为（3.33±1.36）×10^6/kg、（4.14 4±1.67）×10^6/kg、（2.79±1.47）×10^6/kg,第5天达到峰值（P〈0.05）,第4天次之（P〈0.05）,第6天再次之（P〈0.05）.108例供者采集2次（第4天、第5天）均可满足移植标准.所有供者均无严重不良反应发生.结论 单一低剂量G-CSF能够有效动员健康供者造血干细胞.动员后于第4、第5天行干细胞采集优于第5、第6天.

Objective To investigate the mobilization effect of low-does granulocyte-colony stimulation factors（G-CSF） on allogenic peripheral stem cell transplantation （PBSCT） and the best acquisition time for haemopoietic stem cell. Methods G-CSF was administered subcutaneously at 5μg·kg^（-1）·d^（-1）to 108healthy donors. The mobilization effects were assayed by mononuclear cell count,flow cytometry and colonyforming units analysis.Results Onfourth day,fifty day and sixth day, the ratio of CD34^＋ were （0.71 ±0.08）%, （1.09±0.09） %, （0.57±0.08） %, respectively. The CFU-GM yield were （93.33±44.51）/10^5MNC, （124.61±57.85）/ 10^5MNC, （80.25±49.24）/10^5MNC, respectively. The number of CD34^＋ were （3.33±1.36）×10^6/kg, （4.14±1.67） ×10^6/kg, （2.79±1.47）×l0^6/kg, respectively. The ratio and number of CD34^＋ cell of PBSC collections of fifth day was higher than that of the fourth day （P 〈0.05）. And the fourth day was higher than that of the sixth day（P 〈 0.05）. Twice day （fourth day and fifth day） collection can obtain enough CD34^＋ cell for PBSCT. There were no significant adverse effects. Conclusion Low-does G-CSF takes good effects on the mobilization of PBSC. And it was better to collect stem cell on fourth day and fifth day rather than on fifth day and sixth day after mobilization.