拉米夫定与原儿茶酸药物组合对HBV X蛋白入核的影响

Effect of Lamivudine Combined with Protocatechuic Acid on the Distribution of HBV X Protein in Cellular Nucleus

目的研究拉米夫定与原儿茶酸药物组合对乙肝病毒(HBV)X蛋白入核的影响。方法 Huh7细胞转染外源性GFP-HBx融合质粒后,与药物作用一定时间,利用共聚焦荧光显微镜观察药物对外源性HBV X蛋白在细胞内分布的影响。HepG2.2.15细胞与药物作用一定时间后,用Western blot分析药物对内源性HBV X蛋白在细胞核内含量的影响。结果拉米夫定及其与原儿茶酸的药物组合对HBV X蛋白的入核有抑制作用,且联合用药效果最好。结论原儿茶酸能增强拉米夫定对HBV X蛋白入核的抑制,这是该药物组合抑制HBV复制的重要作用途径之一。

OBJECTIVE To investigate the effect of lamivudine（3TC） combined with protocatechuic acid（PA） on the distribution of HBV X protein in cellular nucleus.METHODS Huh7 cell was transfected with ectogenic GFP-HBx fused plasmid,and treated by 3TC,PA and 3TC PA combination respectively the effect of drug treatment on the distribution of HBV X protein（HBx） in Huh7 cell was analyzed by using confocal microscopy.Furthermore,HepG2.2.15 cell was treated by 3TC,PA and 3TC PA combination respectively.The effect of drug treatment on the content of endogenic HBx in cellular nucleus of HepG2.2.15 cell was analyzed by Western blot.RESULTS 3TC and 3TC PA combination inhibited HBx into cellular nucleus,and the inhibitory action of 3TC PA combination was more powerful than that of 3TC.CONCLUSION The inhibitory action of 3TC PA on HBx may be an important mechanism of action on HBV replication.