雷公藤多苷对糖尿病肾病模型肾小球损伤的影响

Preventive effects of multi-glycoside of Tripterygium wilfordii on glomerular lesions in experimental diabetic nephropathy

目的:观察雷公藤多苷(multi-glycoside ofTripterygium wilfordii,GTW)对糖尿病肾病模型肾小球损伤的改善效果。方法:运用链脲佐菌素(streptozotocin,STZ)建立糖尿病肾病模型,以GTW干预,并设立正常、安慰剂以及贝那普利对照组。比较体重、尿微量白蛋白(urine albumin,UAlb)、血糖(blood glucose,BG)、血清肌酐(serum creatinine,Scr)、血清尿素氮(blood urea nitrogen,BUN)以及肾小球形态(细胞总数、细胞外基质)的变化,并检测肾小球中α-平滑肌肌动蛋白(α-smoothmuscle actin,α-SMA),Ⅰ型胶原的表达水平。结果:GTW和贝那普利都可以减少模型鼠UAlb;对于模型鼠肾小球硬化性损伤,如系膜细胞增生,α-SMA和Ⅰ型胶原异常表达等,GTW的作用强于贝那普利,肾小球总细胞数GTW组(54.44±2.41),贝那普利组(67.83±4.41),P<0.05;α-平滑肌肌动蛋白积分GTW组(1.98±0.52),贝那普利组(2.27±0.46),P<0.05;I型胶原积分GTW组(2.11±0.37),贝那普利组(2.88±0.58),P<0.05。结论:GTW改善DN肾小球损伤的效果包括减少蛋白尿和改善肾小球硬化。

Objective：To observe the preventive effects of multi-glycoside of Tripterygium wilfordii（GTW）on glomerular lesions in experimental diabetic nephropathy（DN）.Method：The DN model of rats was established with streptozotocin（STZ）and intervened with GTW.In the same time,normal,benazepril,and vehicle control groups were set up.After 8 weeks of oral treatment with GTW（50 mg·kg-1BW）,benazepril（6 mg·kg-1BW）,and vehicle（shysiological saline）,the changes of body weight,urine albumin（UAlb）,blood glucose（BG）,serum creatinine（Scr）,blood urea nitrogen（BUN）and glomerular morphology were examined.In addition,the level of protein expression of α-smooth muscle actin（α-SMA）and collagen type Ⅰ in glomeruli was determined by immunofluorescence.Result：Both GTW and benazepril reduced UAlb.GTW ameliorated glomerular injury,such as mesangial cell proliferation,α-SMA and collagen type Ⅰover-expression,in DN model.Compared with benazepril,beneficial effects of GTW on glomerulusclerosis were more significant（total cell number：GTW group 54.44±2.41,benazepril group 67.83±4.41,P0.05;α-SMA score：GTW group 1.98±0.52,benazepril group 2.27±0.46,P0.05;collagen typeⅠscore：GTW group 2.11±0.37,benazepril group 2.88±0.58,P0.05）.Conclusion：Preventive effects of GTW on glomerular lesion in DN model are related to decreasing UAlb and ameliorating glomerulusclerosis.