傅里叶变换红外光谱法应用于水-乙醇溶液中海南霉素的测定

Determination of Hainanmycin in the Aqueousalcohol Solution by Fourier Transform Infrared Spectroscopy

随着化学计量学方法处理光谱重叠和光谱采样技术的发展，红外光谱应用于多组份同时定量分析和过程分析的研究日益受到人们的关注［１～５］．海南霉素（Ｃ４７Ｈ７９Ｏ１５Ｎａ，分子量９０７．１２２）是国家一类新药，用于防治鸡球虫病，易溶于有机溶剂，不溶于水．在实...

Fourier transform infrared(FTIR) spectroscopy equipped with horizontal attenuated total reflection(ATR) accessory was applied to the multicomponent quantitative analysis in organic phase. A novel method was developed to directly determine the Hainanmycin(HNMS, an insoluble antibiotic from the fermentation) in water and ethyl alcohol solution. Calibration models were established on the basis of partial least squares with infrared spectra(IR) and secondderivative absorbance spectra(DIR) of the standard mixtures. The better predicted results is obtained especially at lower concentration of HNMS by DIR method. The standard errors of prediction of HNMS by IR or DIR are 018 and 019 g/L, respctively. The standard errors of prediction of water and alcohol are 0001 1 volume ratio by either quantitative method. The method is rapid and convenient without any pretreatment.