漆树漆酶的催化氧化作用 (ⅹⅢ)──Fe^(2+)离子对漆酶催化氧化反应的影响()

Catalyzed Oxidation by \%Rhus Vernicifera\% Laccase() \= Effects of Fe\+\{2+\} Ion on the Catalytic Oxidation by Laccase

以５，６－二溴－２，３－二氰基氢醌为底物，在ｐＨ４．５０条件下，用分光光度法考察了ＦｅＳＯ４对漆树漆酶催化氧化反应的影响．结果表明，在本文实验条件下ＳＯ２－４离子对酶促反应的影响基本可以忽略，而Ｆｅ２＋离子对漆酶的催化氧化反应则有明显的竞争性抑制作用．研究证实。

The effects of FeSO\-4 on the oxidation of 5,6\|dibromo\|2,3\|dicyanohydroquinone(DDBQH\-2) catalyzed by \%Rhus vernicifera\% laccase were studied spectrophotometryly under condition of pH 4\^50. The results show that under the condition of experiments, SO\+\{2-\}\-4 ion has little effect on the enzymatic reaction, but Fe\+\{2+\} ion has obvious inhibition, the nature of inhibition is competitive type. The results demonstrate that the inhibition is realized through the reduction of product of enzymatic reaction by Fe\+\{2+\} ion.