薄层分离-库仑滴定法测定茄尼醇

Determination of Solanesol by Layer Chromatographic Separation-Coulometric Titration

提出以2mol/L KBr-冰醋酸(1:3 V/V)为电解液,在阳极电生溴与茄尼醇发生反应来测定茄尼醇含量的库仑滴定法.茄尼醇与溴反应,其n值为18.对于烟叶提取物中茄尼醇,以正已烷-二氯乙烷-丙酮为展开剂,用薄层色谱法予以分离,用库仑滴定法进行测定.

A method for the determination of solanesol by coulometric titration was developed. The titration was carried out in a mixture of 2 mol/L KBr-glacial acetic acid (1:3 V/V), and bromine was generated at the anode. Solanesol reacted with bromine with a value of n as 18. The solanesol in tobacco extracts was separated by thin layer chromatography with a developer of n-hexane-dichloroethane-acetone and then determined by coulometric titration method.