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毛细管电色谱法测定氨茶碱和鲁米那片剂中茶碱和苯巴比妥的含量 被引量:3

Reversed Phase-Capillary Electrochromatographic Determination of Theophylline and Phenobarbital in Aminophylline and Lumina Tablets
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摘要 首次报道了CEC法进行药物成分测定的应用研究.采用反相毛细管电色谱法,以非那西丁为内标,UV检测波长为214nm,以乙N:Tis-磷酸缓冲液(70:30,V/V,pH2.6)为流动相,在75μm(1.D.)×20cm的毛细管ODS(5μm)填充柱上测定了氨茶碱和鲁米那片剂中茶碱和苯巴比妥的含量,在所测的浓度范围内有良好的线性关系和回收率. The direct determination of the theophylline and phenobarbital in aminophylline and lumina tablets by reversed phase-capillary electrochromatography (RP-CEC) with phenacetin as the internal standard is reported in this paper. The experiment was conducted on 75 μm(I. D. ) × 20 cm and 5 μm ODS packed capillary column, with acetonitrile-tris/phosphoric acid buffer(70/30, pH 2.6) as mobile phase. The detective wavelength of UV detector was at 214 run. Good linear relationship and recovery were obtained in the range of appropriate concentrations.
机构地区 清华大学化学系
出处 《分析化学》 SCIE EI CAS CSCD 北大核心 1999年第1期97-100,共4页 Chinese Journal of Analytical Chemistry
基金 国家自然科学基金重点资助项目
关键词 反相 毛细管电色谱 氨茶碱 苯巴比妥 测定 Reversed phase-capillary electrochromatography, theophylline, phenobarbital
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  • 2路纯明,王仁育.示波极谱法测定茶叶中的茶碱[J].河南工业大学学报(自然科学版),2006,27(4):7-10. 被引量:6
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