光度法对铜-苯丙氨酸配合物组成及稳定常数的测定

SPECTROPHOTOMETRIC STUDIES ON THE COMPOSITIONS AND STABILITY CONSTANTS OF COPPER (Ⅱ) PHENYLALANINE COMPLEXES

铜、苯丙氨酸及二者的配合物在紫外光谱区均有较强的吸收，为求得纯配合物的吸光度，我们建立了三参比法，并在此基础上探讨了ｐＨ值对配合物形成的影响。实验证实当ｐＨ≥７．５时，二者仅形成１∶２（Ｍ∶Ｌ）的配合物。我们还采用对应溶液法测定了该配合物的稳定常数。

Copper (Ⅱ), Phenylalanine (Phe) and their complexes all have strong ultraviolet absorptions In order to obtain the absorbance of Cu(Ⅱ)￣Phe complexes, we set up three reference method, discussed the influence of pH to the formation of the complexes, and justified that when pH≥7 5, only 1∶2 (Cu∶Phe) complex was formed We further detected stability constants of Cu(Ⅱ)￣Phe complexes using corresponding solution method