反相色谱多二元溶剂的选择性调整

Selectivity Tuning in Multi Binary Eluents for Reversed Phase Liquid Chromatography (RPLC)

从统计热力学方法推导的溶质保留规律及其相关参数与分子结构之间的关系式出发，探讨了１６种多环芳烃在甲醇／水、乙腈／水、异丙醇／乙腈３种二元溶剂体系下选择性的差异，为确立复杂化合物分离的溶剂选择原则奠定了基础。

n this article, the retention equation and the relationship between retention parameters and the parameters of molecular structure deduced from statistical thermodynamics in RPLC have been used to explain the difference of selectivity towards a particular species of compounds polycyclic aromatic hydrocarbons (PAHs). Methanol/water, acetonitrile/water and isopropanol/acetonitrile have been provided in advance, then the retention behaviors of sixteen PAHs under three binary solvent systems have been investigated. It is found that each pair of binary solvents of methanol/water, acetonitrile/water and isopropanol/acetonitrile has its own unique selectivity. The best selectivity obtained for acenaphthene and fluorene is methanol/water system for fluoranthene and pyrene is acetonitrile/water, and for benzoperylene and dibenzo anthracene is isopropanol/acetonitrile. So a three stepwise gradient elution of multi binary mobile phase can be chosen for separation of 16 PAHs.