红花罂粟植物的各个部分中蒂巴因含量的测定

Determination of Thebaine in Various Tissues of Papaver Bracteatum by Gas Chromatography

采用甲醇浸泡、超声波一次提取处理样品，气相色谱内标法定量测定不同种类及不同培育方法的红花罂粟植物的全果、根、叶、苞片、果盖、果壳、茎中蒂巴因的含量。方法采用多点校正，其线性相关系数为０．９９６，变异系数为２．９％～５．４％，最低绝对检出量可达２ｎｇ。蒂巴因的色谱峰为双峰，经质谱确证为异构体，这两种异构体的比例不固定，取其峰面积之和定量。应用该方法对前后３年共１６８个样品进行了测定，提取样品中蒂巴因的图谱与蒂巴因标准品的图谱完全一致，样品中的其它杂质均不干扰蒂巴因的测定。

Papaver bracteatum is an important source of opiates because of its high thebaine content. An efficient and convenient method of determining thebaine, not only in the root, but in the more highly pigmented capsule, stem and leaf, is urgently required. We decided to examine these in detail on a wide variety of plant material and to choose an assay method which is convenient and gives reproducible results. A simple method for the quantitative extraction and analysis of thebaine in the different tissues (capsule, root, leaf and stem) of papaver bracteatum has been established. The powdered materials were extracted with methanol under ultrasonic condition. The procedure of extraction is simple, rapid and reliable. The assay method gave satisfactory reproducibility for a wide range of plant materials (coefficient of variation 2.9% to 5.4%). The linear range of response of thebaine was from 5.0 to 500.0 mg/L. The minimum limit of detection was 2 ng. The operating time of the assay is about 20 min. The presence of other alkaloids did not interfere with the assay reported here in.