摘要
报道了用高效液相色谱配合电化学检测器同时分析组织胺(Hist)和去甲肾上腺素(NA)的方法。用邻苯二甲醛(OPA)进行柱前衍生,流动相为V(乙酸钠缓冲溶液)V(乙腈)=73,检测器电位+0.7V,线性范围为0.015~5μg/L(r=0.998),平均回收率为95%。方法灵敏、准确,重复性好,分析速度快,可用于临床检测。
This paper reports a reversed phase high performance liquid chromatographic method for the simultaneous separation and determination of histamine(Hist) and noradrenaline(NA) in human plasma by means of pre column derivatization of Hist and NA with OPA in the presence of 2 mercaptoethanol. The analysis was carried out with Partisil ODS column, V[sodium acetate buffer solution(pH 4.90)]∶V(acetonitrile)=7∶3 as mobile phase and 3 methylhistamine as internal standard. The detector potential was +0.7 V for both compounds to keep minimum background noise. A linear relationship between the electrochemical signal and the compound mass concentrations was obtained. The minimum detectable limit was 15 ng/L (S/N=3) . An example of application of the method to the determination of Hist and NA in human plasma is presented.
出处
《色谱》
CAS
CSCD
北大核心
1999年第1期80-82,共3页
Chinese Journal of Chromatography
基金
河南省医学科学院科研基金
关键词
人血浆
HPLC
测定
组织胺
去甲肾上腺素
high performance liquid chromatography, electrochemical detection, histamine, noradrenaline, pre column derivatization