高效液相色谱-电化学检测法同时测定人血浆中的组织胺和去甲肾上腺素

Simultaneous Analysis of Histamine and Noradrenaline in Human Plasma by High Performance Liquid Chromatography/Electrochemical Detection

报道了用高效液相色谱配合电化学检测器同时分析组织胺（Ｈｉｓｔ）和去甲肾上腺素（ＮＡ）的方法。用邻苯二甲醛（ＯＰＡ）进行柱前衍生，流动相为Ｖ（乙酸钠缓冲溶液）Ｖ（乙腈）＝７３，检测器电位＋０．７Ｖ，线性范围为０．０１５～５μｇ／Ｌ（ｒ＝０．９９８），平均回收率为９５％。方法灵敏、准确，重复性好，分析速度快，可用于临床检测。

This paper reports a reversed phase high performance liquid chromatographic method for the simultaneous separation and determination of histamine(Hist) and noradrenaline(NA) in human plasma by means of pre column derivatization of Hist and NA with OPA in the presence of 2 mercaptoethanol. The analysis was carried out with Partisil ODS column, V[sodium acetate buffer solution(pH 4.90)]∶V(acetonitrile)=7∶3 as mobile phase and 3 methylhistamine as internal standard. The detector potential was +0.7 V for both compounds to keep minimum background noise. A linear relationship between the electrochemical signal and the compound mass concentrations was obtained. The minimum detectable limit was 15 ng/L (S/N=3) . An example of application of the method to the determination of Hist and NA in human plasma is presented.