峰蜜中杀虫脒的快速测定法

Rapid Method for Determination of Chlordimeform Residue in Honey

建立了测定峰蜜中杀虫脒的薄层色谱法，不需要大型仪器和特殊试剂，不受基体干扰，整个测定过程仅需３ｈ，回收率在７０％以上。

In the present work, TLC was used as a rapid method for determination of chlordimeform in honey . Twenty gram of sample was extracted by n hexane in alkali solution, re extracted by acidic aqueous solution, then hydrolyzed in NaOH solution for 10 min on boiling water bath. The hydrolyzed products were extracted by methylene chloride. After passing through the Na 2SO 4 column for dehydration, the methylene chloride should be evaporated to less than 70 ℃ to 0 5 mL spot 50 μL sample solution on the silica gel plate, develop the plate about 8 cm from the surface of developing solvent V (benzene)∶ V (chloroform)∶ V (ethyl acetate)=5∶5∶1. The plate was kept in the atmosphere of (NaNO 2+HCl) for 10 min and 5% N (1 naphthyl) ethylendiamine dihydrochloride was sprayed on it. Put it into a dark room for 20 min and then observe and estimate the amount of chlordimeform in the sample. The limit of determination is 10 ng. The experiment could be completed in 3 h for a sample without large equipment and special reagent.