食管癌患者CTGF基因mRNA表达及预后分析

Expression of CTGF mRNA in human esophageal cancer tissues and survival analysis

目的检测人食管癌组织中结缔组织生长因子(CTGF)基因mRNA和蛋白表达水平,了解食管癌患者术后生存情况及其影响因素。方法运用实时定量PCR技术测定186例食管癌患者的癌组织及配对的癌旁组织中CT-GF基因mRNA和蛋白的表达水平,采用Kaplan-Meier单因素与Cox多元回归进行生存分析。结果食管癌组织中CTGFmRNA表达水平(5.29±5.12)明显高于其配对的癌旁组织(8.63±5.12)(t=10.112,P=0.0000);食管癌组织CTGF蛋白表达水平(20.83±8.20)明显高于其配对的癌旁组织(4.52±4.53)(t=23.820,P=0.0000);Kaplan-Meier法生存分析结果显示,CTGFmRNA及CTGF蛋白的表达水平、性别、家族史、浸润深度、肿瘤大小、淋巴结转移、分化程度与患者的生存率明显相关;Cox回归分析结果显示,CTGFmRNA的表达水平、CTGF蛋白的表达水平、性别、家族史、淋巴结转移、分化程度是影响食管癌患者生存率的独立危险因素。结论食管癌组织中CTGF的高表达可能影响食管癌患者术后的生存,其可作为食管癌患者预后的一个评价指标,并可能成为基因治疗的一个作用靶点。

Objective To investigate connective tissue growth factor（CTGF） mRNA expression level in esophageal cancer and to analyze the influencing factors of the survival of esophageal cancer patients after operation.Methods We quantified CTGF mRNA expression level and CTGF protein expression level in 186 pairs of esophageal cancer and normal esophageal tissues with real-time PCR assay.Results Paired-sample t-test indicated that the expression level of CTGF mRNA in cancers （5.29±5.12）was significantly higher than that of in matched controls（8.63±5.12,t=10.112,P=0.0000） and the expression of CTGF protein level in cancers （20.83±8.20）was significantly higher than that of in matched controls（4.52±4.53,t=23.820,P=0.0000）.The univariate survival analysis showed that the mRNA expression of CTGF,the protein expression of CTGF,gender,family history,metastasis,and differentiation had significant association with survival rate.Cox regression survival analysis showed that the mRNA expression of CTGF,the protein expression of CTGF,gender,family history,lymphonode metastasis,differentiation,infiltration depth,tumor size were significantly independent factors of survival rate.Conclusion Up-regulation of CTGF in esophageal cancer may influence patient’s prognosis and it may be identified as a novel prognostic indicator and a potential therapeutic target.