三氯乙烯对大鼠睾丸细胞凋亡及基因表达影响

Effects of trichloroethylene on apoptosis and proto-oncogene expression in male rat testis spermatogenetic cells

目的探讨三氯乙烯对大鼠睾丸细胞凋亡、c-myc和p53表达的影响。方法选用雄性SD大鼠15只,随机分为3组,每组5只,实验组用1500和3000mg/kg的三氯乙烯经口灌胃染毒,对照组给予等容量花生油。流式细胞术检测细胞凋亡和细胞增殖,用RT-PCR检测c-myc和p53表达。结果 1500和3000mg/kg组大鼠睾丸细胞凋亡率分别为22.11%和25.96%,明显高于对照组(P<0.01);增殖指数分别为15.51±0.89和13.91±1.02,明显低于对照组(P<0.05),睾丸细胞c-mycmRNA分别为0.698±0.067和0.837±0.062,p53mRNA分别为0.619±0.109和0.761±0.094,均较对照组明显增高(P<0.01)。结论三氯乙烯能明显诱导大鼠睾丸细胞凋亡并抑制增殖,并使睾丸细胞c-myc和p53的表达增强。

Objective To study the effects of trichloroethylene on apoptosis,c-myc and p53 expression in rat spermatogenetic cells.Methods Male SD rats were divided into 3 groups and there were 5 rats in each group.Trichloroethylene at the doses of 1 500 and 3 000 mg/kg was given to the rats by gavage.Apoptosis was measured with flow cytometry.c-myc and p53 expression in rat spermatogenetic cells were measured with RT-PCR.Results The apoptosis rate of spermatogenetic cells in rat testis were 22.11±2.29 and 25.96±1.74 at the doses of 1 500 and 3 000 mg/kg trichloroethylene,respectively,with significant differences from that of the control（5.59±1.89,P0.01）.Proliferation index of spermatogenetic cells were 15.51±0.89 and 13.91±1.02,respectively,and lower than that of control（20.78±3.52） significantly.Trichloroethylene at those doses also increased proto-oncogene c-myc and p53 expression obviously in rat testis spermatogenetic cells（P0.01）.c-myc mRNA were 0.698±0.067 and 0.837±0.062,and p53 mRNA were 0.619±0.109 and 0.761±0.094 at the doses of 1500 and 3 000mg/kg trichloroethylene,respectively.Conclusion Trichloroethylene induces apoptosis,inhibits proliferation and causes overexpression of c-myc and p53 in rat testis spermatogenetic cells.