光合细菌球形红杆菌对活性艳红X-3B的脱色作用及其与质粒的关系

Decolorization of Rhodobacter Sphacroides on Reactive Red X-3B and it's Plasmid

本文研究了光合细菌球形红杆菌在不同条件下对活性艳红Ｘ－３Ｂ的脱色效果及与质粒的关系。结果表明，球形红杆菌的培养条件对脱色效果有较大的影响，ｐＨ在６～９范围内２４ｈ脱色率均在８５％以上，最适ｐＨ稍偏碱为７～９；脱色的最适温度范围较宽，在１５℃～４０℃之间２４ｈ脱色率均在７５％以上；氧的存在对脱色是不利的，厌氧条件下的脱色效果远远高于好氧条件下的脱色效果。结果还表明，该菌株质粒在琼脂糖凝胶电泳图谱上仅有一条质粒带。通过高温吖啶橙处理，消除该菌株的质粒后，该菌株的脱色能力也随之消失，从而证明该菌株的脱色能力是由质粒控制的。

This paper studied the influence of three factors on decolorization and the relation between decolorization and plasmid.The results showed that cultural conditions greatly influenced decolorization rates.In pH6～9,the decolorization rates were over 85%and at 15℃～40℃,the decolorization rates were over 75%.The decolorization rates in anaerobic condition were higher than that in aerobic condition.By using the agar gel electrophoresis technique,it is found that the strain has a plasmid and fails to decolorize Reactive Red X-3B after removing its plasmids.These results showed the dye decolorizing ability of Rhodobacter sphacroides was controlled by its plasmids.