复合菌种协同发酵酒糟生产饲料蛋白研究

A Study on Feed Protein Producting from Distillers' Grains by Multiple Bacteria Synergistic Fermentation *

以笔者选育的８５０３和８５０５复合菌种为试验菌，以白酒糟为原料，经最适条件试验．确定最优条件为：培养温度３０．０℃，初始ｐＨ５．５，（ＮＨ４）２ＳＯ４添加量为５ｍｇ／ｍｌ，投料量１０％，接种量５％—１０％，发酵期限５ｄ．在最适条件试验的基础上，进行了５Ｌ发酵罐试验，发酵产物粗蛋白质含量由２３．７５％提高到３５．７５％，提高了１１．００％，其中真蛋白质提高１０．３４％，粗纤维降低了２．０５％，氨基酸总量由２５．３５％提高到４０．６８％，提高了１５．３３％．

The bacterium 8503 and 8505,which have been seletively bred in the laboratory,are regarded as experimental bacteria and distillers'grains as sole raw material.through single factor experiments,the best fermentation condition to produce feed protein from distillers'gains are as follows,culture temperature 30 0℃,initial pH5—6,(NH 4) 2SO 4 5mg/ml,inoculum size 5%—10%,raw material 10%,fermentation time 5 days.According to single factor experiment about the best fermentation condition,experiment in a 5L fermenter shows that on the condition without adding other raw materials the raw protein in distillers grains are raised up to 35 75% from 23 75%,among that the pure protein is 11% higher than the original distiller's grains,and the content of fiber is 2.05% less than in the original,and total quantity of amine acid are raised up to 40.68% from 25 35%.It is 15.33% higher than the original distiller's grains.