肉中单核细胞增生李斯特菌PCR快速检测方法建立

Polymerase Chain Reaction Method for Detection of Listeria monocytogenes in Meat Samples

目的:建立单核细胞增生李斯特菌(Listeria monocytogenes,LM)快速、敏感、特异的PCR诊断方法。方法:采用聚合酶链式反应技术(PCR)特异性扩增单核细胞增生李斯特菌内化素基因(ivlA),并评价该方法的特异性与敏感性。结果:在445bp处出现inlA基因的目的片断,只有单增李斯特菌的目的片段获得扩增,其他菌种扩增均呈阴性;该方法可以检测到DNA的检测限。结论:PCR方法比传统细菌检测方法更特异、快速、灵敏和简便;为肉中单增李斯特菌的快速检测提供了新的手段。

Objective：To establish a rapid, sensitive and specific polymerase chain reaction （PCR） method for detection of Listeria monocytogenes （LM）. Methods ： Polymerase chain reaction （PCR） technique was used to amplify the ivlA gene of Listeria monocytogenes of food specifically,value the specificity and sensitivity of this method. Results：The target gene ivlA was detected at 445bp, and only the target gene of Listeria monocytogenes was amplified while the other bacteria was not detected; this method could also detect the limit of DNA. Conclusion：The PCR method is more concise, rapid, sensitive and more specific than conventional culture method for detection of Listeria monocytogenes in meat samples. This method is useful for the investigation of Listeria in meat samples.