摘要
采用硫酸铵分级沉淀结合离子交换层析,从鲶鱼体表粘液中分离纯化IgM。实验结果表明,离子交换层析分离最佳洗脱pH为7.4、洗脱速度为3mL/min、NaCl浓度为1mol/L。SDS-PAGE测定IgM重链和轻链分子量分别为78ku和26ku。通过小鼠脾淋巴细胞增殖实验得知IgM的浓度为6mg/mL时具有较强的免疫调节活性。
This experiment extract and purify IgM from the mucus of catfish by means of gradient sediment using ammonium sulfate and ion-exchanged Chromatography.The results showed that the best pH for ion-exchanged chromatography was 7.4, the best velocity was 3ml/min, and the concentration of NaCl was lmol/L. The relative molecular mass of the heavy chaine and light chain of IgM was 78ku and 26ku,respectively,as determined by SDS-PAGE.Trough the experiment of rat Splenic lymphoid cell proliferation, we know that when the concentration of IgM was 6 mg/mL, its immunoregulatory activity was better.
出处
《肉类研究》
2010年第4期56-59,共4页
Meat Research
关键词
鲶鱼
粘液
IGM
分离纯化
免疫活性
catfish
mucus
immunoglobulin M
extraction and purification
immunoregulatory activity
