秋水仙碱对斑马鱼肝脏和鳃组织中SOD及Na^+-K^+-ATPase活性的影响

Effects of Colchicine on the Activities of SOD and Na^+-K^+-ATPase in the Liver and Branchi of Brachyclanio rerio

将斑马鱼(Brachyclanio rerio)暴露于秋水仙碱5个浓度组(0mg·mL-1、10.00mg·mL-1、14.12mg·mL-1、19.95mg·mL-1、28.18mg·mL-1、39.80mg·mL-1)中进行96h急性毒性试验,计算其半数致死浓度(LC50),再设置秋水仙碱3个浓度(0.67mg·mL-1、1.70mg·mL-1、4.26mg·mL-1)将斑马鱼进行21d慢性毒性实验,每7天测定肝脏和鳃中的超氧化物歧化酶(SOD)及N+-K+-三磷酸腺苷酶(Na+-K+-ATPase)活性。结果表明,秋水仙碱对斑马鱼的LC50为16.90mg·mL-1,随着秋水仙碱浓度增大和染毒时间延长,斑马鱼鳃中SOD和Na+-K+-ATPase活性均显著受到抑制,肝脏中SOD活性增加,Na+-K+-ATPase活性总体呈现抑制趋势。斑马鱼肝脏和鳃中的SOD及Na+-K+-ATPase对秋水仙碱敏感,可以作为观测指标用于评价秋水仙碱的毒性。

Braehyclanio rerio were exposed to five different concentrations of colchicine for 96h, and the median lethal concentration （LCso） was calculated for evaluating acute toxicity. In addition, Brachyclanio rerio were exposed to three different concentrations of colehicine （0.67, 1.70, 4.26mg.L^-1） for 21 days in chronic toxicity test, and the activities of SOD and Na^＋-K^＋-ATPase in the liver and branchi of Brachyclanio rerio were measured at interval of 7 days. The result indicates that LC50 of colchicine was 16.90mg·L^-1 to Brachyclanio rerio. With the increases of colchicine concentrations and exposure time, the activities of SOD and Na^＋-K^＋-ATPase in the branchi of Brachyclanio rerio were suppressed significantly, and the activity of SOD in liver was increased, but the activity of Na＋-K＋-ATPase in liver presented the tendency of depression. The SOD and Na^＋-K^＋-ATPase in liver and branchi tissue of Brachyclanio rerio are sensitive to toxicant, and can be used as observation index for drug toxicity evaluation.