感染大肠埃希菌后家蝇幼虫血细胞来源的初步研究

Preliminary study on the source of blood cells in Musca domestica larvae infected with Escherichia coli

目的探讨家蝇3龄幼虫感染大肠埃希菌后大量增加的血细胞来源。方法观察对照组及感染大肠埃希菌后4、8、16h组家蝇3龄幼虫血淋巴的血细胞总数(THC)变化;用流式细胞仪检测感染前后各组家蝇3龄幼虫血细胞周期的变化。结果感染后4、8、16h组THC与对照组比较均有显著增加,感染后8 h组THC最高,达(38 920±2274)/μl;对照组幼虫血细胞增殖指数(PI)为15.98%;感染后各组G2/M期和S期细胞增多,PI值均明显增加;感染后4 h组PI值最高,达44.34%。结论家蝇幼虫血细胞自身具有分裂能力,是其造血功能的重要补充。

Objective The source of noticeably increased blood cells in third-instar Musca domestica larvae infected with Escherichia coli（E.coli） was investigated.Methods Changes in the total hemolymph counts（THC） of the third-instar M.domestica larvae infected with E.coli in 4,8 and 16 h and those of the control group were followed and compared.A flow cytometry was used to identify the changes in the blood cell cycle of the third-instar larvae of each group before and after infection.Results The THCs in 4,8 and 16 h after infection were significantly elevated compared with that in the control group.Peaking in 8 h after infection,the highest THC reached（38 920±2274）/μl.The blood cell proliferation index（PI） of the control group was 15.98%.After infection,the G2/M phase and S phase cells increased in each group with significantly increased PI values.Peaking in 4 h after infection,the highest PI value reached 44.34%.Conclusion The blood cells of M.domestica larvae have the ability to divide as a supplement to the hematopoietic function.