浙江省蜱中巴尔通体感染的分子流行病学调查

Molecular epidemiological investigation of Bartonella infection in ticks in Zhejiang province

目的了解浙江省的优势蜱种,检测其巴尔通体感染情况,为巴尔通体的预防控制提供科学依据。方法从天台、金东和江山地区的动物体表采集饱血成蜱,用PCR方法检测其阳性率,并克隆测序后分析所检测到的巴尔通体种类。结果所捕获的蜱均为中华硬蜱,天台、金东和江山地区的阳性率分别为42.3%(11/26)、6.7%(2/30)和3.3%(1/30),平均阳性率为16.3%,所检测到的巴尔通体与Bartonella rattimassilensis的遗传关系最近,与人类致病性巴尔通体B.grahamii的遗传关系也很近。结论浙江省蜱中存在巴尔通体感染,具有传播给人的风险,应该采取措施加以控制。

Objective The dominant species of ticks in Zhejiang province was identified and detected for Bartonella infection in order to provide the basis for the control and prevention of Bartonella human infection.Methods Engorged adult ticks were collected from the body surface of the animals in Tiantai,Jindong and Jiangshan areas,and the prevalence of Bartonella was detected using PCR.Sequencing analysis was then performed to identify the Bartonella species.Results All captured ticks were Ixodes sinensis,with the positive rates of 42.3%（11/26）,6.7%（2/30） and 3.3%（1/30） in Tiantai,Jindong and Jiangshan areas,respectively.The average positive rate was 16.3%.The detected Bartonella had the closest genetic relationship with Bartonella rattimassilensis,followed by the human pathogenic B.grahamii.Conclusion The presence of Bartonella infection in ticks in Zhejiang posed the risk of transmission to humans,and hence the corresponding control measures should be taken.