HPLC法同时测定抗敏颗粒中芍药苷、毛蕊异黄酮苷和阿魏酸的含量

Simultaneous Determination of Peoniflorin, Campanulin and Ferulic Acid in Kangmin Granules by HPLC

目的:建立以高效液相色谱法同时测定抗敏颗粒中芍药苷、毛蕊异黄酮苷和阿魏酸含量的方法。方法:色谱柱为KromasiL C18(250mm×4.6mm,5μm),流动相为甲醇-乙腈-0.4%磷酸溶液(20∶10∶70),流速为1.0mL·min-1,检测波长为254nm,柱温为室温,进样量为10μL。结果:芍药苷、毛蕊异黄酮苷和阿魏酸的检测浓度分别在180～3600μg·mL-1(r=0.9992)、4.6～92.0μg·mL-1(r=0.9991)和8.0～160.0μg·mL-1(r=0.9994)范围内与各自峰面积积分值呈良好线性关系;平均回收率分别为98.3%(RSD=1.8%,n=9)、99.0%(RSD=2.0%,n=9)和100.5%(RSD=1.1%,n=9)。结论:本法操作简便、准确、灵敏、重现性好,可用于抗敏颗粒的质量控制。

OBJECTIVE： To establish an HPLC method for simultaneous determination of peoniflorin,calycosin and ferulic acid in Kangmin granules.METHODS： The separation was performed on a Kromasil C18 column （250 mm×4.6 mm,5 μm）,and the mobile phase consisted of methanol-acetonitrile-0.4% phosphonic acid （20 ∶ 10 ∶ 70） with flow rate of 1.0 mL·min-1.The detection was performed at wavelength of 254 nm.Column temperature was room temperature and the injection volume was 10 μL.RESULTS： The linear ranges of peoniflorin,calycosin and ferulic acid were 180～3 600 μg·mL-1（r=0.999 2）,4.6～92.0 μg·mL-1（r=0.999 1） and 8.0～160.0 μg·mL-1（r=0.999 4）,respectively.The average recoveries were 98.3%（RSD=1.8%,n=9）,99.0% （RSD=2.0%,n=9） and 100.5%（RSD=1.1%,n=9）,respectively.CONCLUSION： The method is simple,accurate,sensitive and reproducible for the quality control of Kangmin granules.