甜茶质量标准研究

Study on Quality Standard of Lithocarpus polystachys

目的:建立甜茶的质量标准。方法:采用聚酰胺薄层色谱(TLC)法对甜茶药材进行定性鉴别;采用紫外分光光度法测定甜茶中总黄酮的含量;采用高效液相色谱(HPLC)法测定甜茶中根皮苷的含量。结果:聚酰胺TLC分离效果良好,斑点清晰。根皮苷最大吸收波长为284nm,6批药材的总黄酮含量(以根皮苷计)在103.12～183.54mg·g-1之间。根皮苷的进样量在0.0998～1.1976μg范围内与峰面积积分值呈良好线性关系(r=0.9997);平均回收率为97.23%,RSD=1.57%(n=6)。结论:所建标准有利于对甜茶的质量控制。

OBJECTIVE： To establish the quality standard of Lithocarpus polystachys.METHODS： TLC was used for the qualitative identification of L.polystachys.The content of total flavonoids of L.polystachys was determined by UV spectrophotometry and the content of phloridzin was determined by HPLC.RESULTS： The TLC spots of polyamide were clear and well separated.The maximum absorption wavelength of phlorizin was 284 nm.The content of total flavonoids of 6 batches of L.polystachys ranged from 103.12 mg·g-1 to 183.54 mg·g-1.The linear ranges of phlorizin were 0.099 8～1.197 6 μg （r=0.999 7） with an average recovery of 97.23%（RSD=1.57%,n=6）.CONCLUSION： Established quality standard is applicable for the quality control of L.polystachys.