HPLC法测定妇良胶囊中芍药苷的含量

Content Determination of Paeoniflorin in Fuliang Capsules by HPLC

目的:建立以高效液相色谱法测定妇良胶囊中芍药苷含量的方法。方法:色谱柱为Shim-pack VP-ODS C18(150mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(15∶85),检测波长为230nm,流速为1.0mL·min-1。结果:芍药苷检测浓度在12.84～102.72μg·mL-1范围内与峰面积积分值呈良好线性关系(r=0.9997);平均回收率为100.18%,RSD=1.46%(n=6)。结论:本方法操作简便、灵敏、准确,可用于妇良胶囊的质量控制。

OBJECTIVE： To establish HPLC method for the content determination of paeoniflorin in Fuliang capsules.METHODS： HPLC was carried out on Shim-pack VP-ODS C18（150 mm×4.6 mm,5 μm） column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution（15 ∶ 85）.The detection wavelength was set at 230 nm at flow rate of 1.0 mL·min-1.RESULTS： The linear range of paeoniflorin were 12.84～102.72 μg·mL-1（r=0.999 7） with an average recovery of 100.18%（RSD=1.46%,n=6）.CONCLUSION： The method is simple,sensitive and accurate for quality control of Fuliang capsules.