细胞色素bc_1复合物结晶的偏振拉曼光谱研究

Polarization Raman Spectroscopic Investigation of Cytochrome bc 1 Complex Crystals

本文报道了用５１４．５ｎｍ和４１３．１ｎｍ激光激发的细胞色素ｂｃ１复合物结晶的偏振显微共振拉曼光谱和用１０６４ｎｍ激光激发的近红外傅里叶变换偏振拉曼光谱。已观察到不同结晶取向和偏振的影响。结果表明，这些取向排列和偏振的影响和分子的对称性质和结晶的三维结构有关。它可能随着三维结构中子单元和还原中心的部位不同而改变。其对称性的不同与三维结构的不对称排列有关，也可归因于蛋白质环境电学上的不对称性。

The polarization micro resonance Raman spectra of cytochrome bc 1 complex crystals with 514.5nm and 413.1nm laser excitation respectively and polarization near infrared FT Raman spectra of bc 1 complex crystals with 1064nm laser excitation are reported in this paper.Distinctive orientation and polarization effects are observed.The results indicated that these orientation and polarization effects are associated with the symmetry properties of the molecular and three dimensional structure of bc 1 complex.It may be varies with the locations of the subunits and the redox centers in the three dimensinal model.The symmetry change may be due to the asymmetric disposition of the porphyrin substituents or to electrostatic asymmetry in the protein surroundings.