摘要
采用反相高效液相色谱技术，以μ－ＢｏｎｄａｐａｋＣ１８作固定相，ＣＨ３ＯＨ／ＴＨＦ／Ｈ２Ｏ作流动相，对鱼油中的二十碳五烯酸（ＥＰＡ）和二十二碳六烯酸（ＤＨＡ）进行了分离纯化，制备溶液经减压蒸馏，乙酸乙酯萃取后所得ＥＰＡ和ＤＨＡ质量分数可分别达到９８％和８６％以上。
In this paper, Preparative HPLC method for EPA and DHA in fish oil was studied. Using fatty acid ethyl esters asrawmaterials,the composition of mobile phase of methanol/tetrahydrofuran/water was optimized.Under appropriate chromatographic conditions and cutting interval, a product containing 98.35% EPA or 86.59% DHA could be obtained, the recoveries were 57.72% and 52.30% respectively.Distillation and solvent extraction methods of HPLC fractions were studied also.
出处
《分析试验室》
CAS
CSCD
1999年第1期42-45,共4页
Chinese Journal of Analysis Laboratory
关键词
鱼油
二十碳五烯酸
二十二碳六烯酸
HPLC
制备
Fish oil
Eicosapentaenoic acid
decosahexaenoic acid,preparative high performance liquid chromatography
