甘草酸二铵对脊髓缺血再灌注损伤后IL-4/IL-8/TNF-α表达的影响

Effects of diammonium glycyrrhizinate on expression of IL-4/IL-8/TNF-α in rats with spinal cord ischemia-reperfusion injury

目的初步探讨甘草酸二铵(Diammonium Glycyrrhizinate,DG)对脊髓缺血再灌注损伤后脊髓组织中炎性细胞因子IL-4、IL-8、TNF-α表达的影响。方法雄性SD大鼠90只,随机分为空白对照组、阴性对照组、DG干预组三组,通过外科手术法造成大鼠腰段脊髓的缺血再灌注损伤,组织匀浆后采用ELISA检测IL-4、IL-8、TNF-α的表达。结果与阴性对照组比较:空白对照组IL-4在24、72、168h有统计学意义(P<0.05),DG干预组IL-4在3、24h时间段有统计学意义(P<0.05);阴性对照组IL-8各时间段均有统计学意义(P<0.05);DG干预组IL-824、72h时间段有统计学意义(P<0.05);空白对照组TNF-α各时间段均有统计学意义(P<0.05);DG干预组TNF-α全部时间段均有统计学意义(P<0.05)。结论脊髓缺血再灌注损伤后IL-4、IL-8、TNF-α的表达明显发生变化,DG可能通过影响IL-4、IL-8、TNF-α的表达发挥保护作用。

Objective To evaluate effects of diammonium glycyrrhiryzinate on ischemia-reperfusion injury of spinal cord and it＇s influence on the expression of IL-4,IL-8 as well as TNF-α in spinal cord tissue.Methods Ninety male Sprauge-Dawley rats were randomly divided into the control group（n=10）,negative control group（n=40）and DG intervention group（n=40）.The spinal cord ischemia-reperfusion model was established by surge method and assay the TNF-α,IL-4,IL-8 levels by ELISA.Results Comparing with the blank group,the IL-4 of negative control group at 24h,72h,168h time period was significantly different（P0.05）,and the DG intervention group in 3h,24h was significantly different（P0.05）;The IL-8 of negative control was significantly different in each period（P0.05）,and the IL-8 of DG intervention group was significant different at 24h and 72h time period（P0.05）;The TNF-a of negative control group was significantly different at each time section（P0.05）,and the DG intervention group were significant different at all the time（P0.05）.Conclusion Significant changes with regard to the expression of IL-4,IL-8 and TNF-α in lumbar spinal cord is found after ischemia-reperfusion injury,and DG may protect the spinal cord by influencing the IL-4,IL-8,TNF-αexpression.