新型酶固定化技术研究进展

Research Progress in Newly-developed Enzyme Immobilization Techniques

酶的固定化技术是酶工程的核心技术之一,它的出现降低了酶制剂的成本,促进了酶的工业化应用。对新型的酶固定化技术进行了综述,包括共价固定法、氨基酸置换法、抗体偶联法、生物素-亲和素亲和法、酶与金属离子的连接和疏水定向固定法及其他技术处理方法,并对固定化技术的应用前景进行了展望。

Enzyme immobilization technology is one of the core technology of enzyme engineering. Such technology could reduce the production cost of zymin and advance industrial application of enzyme. In this paper, the newly-developed enzyme immobilization techniques were introduced including covalent immobilization, amino acid replacement method, antibody coupling method, biotin-avidin system method, connection of enzyme metal ions, hydrophobic oriented immobilization method etc. Besides, the application prospects of immobilization techniques were described.