摘要
目的研究多发性骨髓瘤(MM)患者中miR-342-3p及靶基因EVL的表达情况及意义。方法建立应用颈环式结构引物反转录扩增微小RNA(miRNA)的反转录-聚合酶链反应(RT-PCR)方法 ,并采用荧光定量聚合酶链反应(RQ-PCR)对miR-342-3p及靶基因EVL的表达进行定量分析。结果①5例MM患者中miR-342-3p相对表达量为7.6±5.7,与对照组比较差异有统计学意义(P<0.05)。②MM细胞株U266中miR-342-3p相对表达量为7.0±3.2,较对照组表达增加,差异有统计学意义(P<0.05)。③5例MM患者中EVL基因相对表达量为6.3±3.2,较对照组表达增加,差异有统计学意义(P<0.05)。④MM细胞株U266中EVL基因相对表达量为4.3±1.1,较对照组表达增加,差异有统计学意义(P<0.05)。结论 MM存在miR-342-3p及EVL基因表达异常,可能为研究MM发病机制提供思路。
Objective To investigate the clinical value of expression of miRNA-342-3p and host gene EVL in patients with multiple myeloma（MM） and MM cell line U266.Methods The strain-loop primer was used to reverse transcription and expression of microRNA-342-3p and host EVL gene was quantified by real-time quantitative PCR（RQ-PCR）.Results The relative quantity of miR-342-3p in primary patients with MM and MM cell line U266 was 7.6±5.7 and 7.0±3.2 respectively.MiR-342-3p was overexpressed in MM with respect to normal monuclears.The relative quantity of host EVL gene in primary patients with MM and MM cell line was 6.3±3.2,4.3±1.1.Host EVL gene was overexpressed in MM with respect to normal moleculars.Conclusion It suggests that EVL/miR-342-3p may play a biological role in the mechanism of MM.
基金
深圳市卫生局重点资金资助项目(200901009)
