甲状腺肿瘤中C-met蛋白表达与血管生成及预后的关系

Relationship between C-met expression in thyroid neoplasm and tumor angiogenesis

目的探讨C-met蛋白表达在甲状腺肿瘤血管生成中的作用及其临床意义。方法采用即用型二步法免疫组化检测53例甲状腺肿瘤组织中C-met和CD34的表达情况。结果甲状腺癌组织中C-met蛋白表达阳性率为92.5%,甲状腺腺瘤C-met蛋白表达阳性率为22.7%,在正常组织中未见阳性表达(P<0.01)。微血管密度在C-met表达阳性者明显高于C-met表达阴性者,两者间差异有显著性意义(P<0.05)。甲状腺癌的微血管密度值与组织类型、分化程度、淋巴结转移和肿瘤分期有关(P<0.05),而与患者性别、年龄和肿瘤大小无关。结论原癌基因C-met与甲状腺肿瘤的发生发展密切相关,既可以促进肿瘤细胞的增殖,又可以诱导肿瘤细胞血管形成,与甲状腺癌预后不良有重要关系。

Objective To study the role of oncogene C-met in the process of tumor angiogenesis of thyroid neoplasm and their clinical significance. Methods Immunochemical technique was used to examine C-met and CD34 expression in 53 cases of thyroid neoplasm tissues. Results The positive expression of C-met oncogene protein in thyroid carcinoma was 92.5%, while it was 22.7% in thyroid adenoma, and it was negative in normal thyroid tissue （P 〈0.01）. Microvessel density was significantly higher in C-met positive group than in C-met negative group （P 〈0.05）. The microvessel density value of thyroid carcinoma significantly correlated with histologic classification, differentiation degree, lymph node metastasis and the stage of the tumor （P 〈0.05） and it did not correlate with gender, age and size of tumor. Conclusion Oncogene C-met may be involved in the genesis and development of endometrial adenocarcinoma. It may prompt cellular proliferation and induce tumor angiogenesis and correlate with poor prognosis.